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Anti-160 kDa Neurofilament Medium Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | 160 kDa Neurofilament Medium rabbit polyclonal antibody |
Gene Name: | Neurofilament medium polypeptide |
Synonyms: | 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm |
Immunogen: | Recombinant protein corresponding to Mouse 160 kDa Neurofilament Medium |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. | 96 kDa |
Observed MW. | 150 kDa |
Uniprot ID: | P07197, P08553, P12839 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, spinal cord |
IHC/IF | Mouse, Rat | 1: 500-1: 3000 | brain, spinal cord |
Background
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Images
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Western blot analysis of NF-M (GB111245) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded mouse brain using NF-M (GB111245) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse spinal cord using NF-M (GB111245) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using NF-M (GB111245) at dilution of 1: 1500 (400x lens) |
| Immunohistochemistry of paraffin embedded rat spinal cord using NF-M (GB111245) at dilution of 1: 1500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse brain using NF-M (GB111245) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded mouse spinal cord using NF-M (GB111245) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded rat brain using NF-M (GB111245) at dilution of 1: 500 (400x lens) |
| Immunofluorescence of paraffin embedded rat spinal cord using NF-M (GB111245) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
