ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (100 μl)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (100 μl)

Brand: Abclonal
Cat. #: AS053
Availability: In Stock
$35.00
-+
Applications: IF/IC, FC    
Host Species: Goat    
Isotype: IgG    
Conjugation: ABflo® 488. Ex:491nm. Em:516nm.    
Immunogen: Rabbit IgG
Dilution: IF/IC 1:100-1:500; FC 1:100-1:500
Purification Method: Affinity purification
Concentration: 1.02 mg/mL
Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual




Images

488-conjugated Goat anti-Rabbit IgG (H+L)Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS053) at 1:200 dilution.
Blue: DAPI for nuclear staining.
Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb (5 μl/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L)(AS053, 1:500)was used as a secondary antibody.

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