5-FAM-X-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The X linker between FAM and dUTP can improve the incorporation efficiency.
Key Features
• Bright signals with low background.
• Wide variety of fluorophores.
• Great for many applications, like Fluorescence in situ Hybridization, TUNEL and more.
Applications
Enzymatic non-radioactive labeling of DNA during cDNA synthesis, PCR, nick-translation, random primed labeling, or primer extension.
Specifications
1. Ex/Em: 495/518 nm
2. Molecular Formula: C39H38N4Na3O21P3
3. Molecular Weight: 1060.62
Component
1. 5-FAM-X-dUTP: 25 μl, 1 mM in TE buffer
Storage
Store at -20°C