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Applications: | IF/IC, FC |
Host Species: | Goat |
Isotype: | IgG |
Conjugation: | ABflo® 647. Ex:648nm. Em:664nm. |
Immunogen: | Rabbit IgG |
Dilution: | IF/IC 1:100-1:500; FC 1:500-1:2000 |
Purification Method: | Affinity purification |
Concentration: | 1.02 mg/mL |
Buffer: | PBS with 0.02% sodium azide,1%BSA,50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual |
Images
![]() | Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (A24996,dilution 1:200) followed by a further incubation with ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L) (AS060, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
![]() | Flow cytometry: 1X10^6 K-562 cells (negative control,left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test,orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 647-conjugated Goat Anti-Rabbit IgG (H+L)(AS060, 1:2000) was used as a secondary antibody. |