AAV CAG-iCre (30 µl)

Cre recombinase is a type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals. By optimizing the mammalian codon usage for Cre recombinase, Cre expression is improved in the mammalian cells. This codon-improved Cre (known as iCre) transgene shows lower CpG content from the prokaryotic coding sequence, thus reducing the chances of epigenetic silencing in mammals.

AAV9-CAG-iCre is a pre-made rAAV in serotype 9 (with capsid from AAV9 and 2xITR from AAV2) which expresses iCre recombinase under CAG promoter (also known as CBA promoter). This product used in the Cre-lox system as a genetic tool to generate site-specific recombination of DNA between loxP sites in cultured cells and animal experiments.