AAV CMV-FLPo (30 µl)

FLP originally identified from Saccharomyces cerevisiae is a site-specific recombinases (SSR) from the ϧ integrase family and recognizes distinct 34 bp FRT sequence. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe which was 4-fold increase in recombination efficiency at 37 °C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin.

FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This AAV9-CMV-FLPo pre-packaged rAAV in serotype 9 (with capsid from AAV serotype 9 and 2xITR from AAV serotype 2) will over-express FLPo recombinase under CMV promoter. Ready to use format.