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Anti-alpha smooth muscle Actin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R & M |
| Applications: | WB & IHC/IF & IEM |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | alpha smooth muscle Actin rabbit polyclonal antibody |
Gene Name: | Actin, aortic smooth muscle |
Synonyms: | Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46, SMA, a-sma, sma |
Uniprot ID: | P62736, P62737, P62738 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse α smooth muscle actin |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Predicted MW. | 42 kDa |
Observed MW. | 42 kDa |
Product Usage Information
| Applications | Species | Dilution | Positive Sample |
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 | heart, liver |
| IHC/IF | Human, Mouse, Rat | 1: 300-1: 1000< | breast cancer, heart, liver, lung, lung cancer, brain, stomach |
| IEM | Mouse | 1: 50 | aorta |
Background
Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA). Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.Alpha-smooth muscle actin (α-SMA) is commonly used as a marker of myofibroblast formation.
Images
| Western blot analysis of alpha smooth muscle Actin (GB111364) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human lung cancer using Actin (GB111364) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse heart using Actin (GB111364) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse lung using Actin (GB111364) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded rat lung using Actin (GB111364) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed human breast cancer using Actin (GB111364) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using Actin (GB111364) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat heart using Actin (GB111364) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat liver using Actin (GB111364) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed human para-cancer lung using COLIII (GB11629) (green) + Collagen I (GB11022-3) (red) + Actin (GB111364) (yellow) + CD31 (GB12064) (orange) at dilution of 1: 300 |
| Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using Actin(GB111364) (green) + CD31(GB12064) (spred) + CD3(GB11014) (pink) at dilution of 1: 300 |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse aorta using Actin (GB111364) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50 |
Storage
Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
