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Anti-Iba1 Mouse mAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Allograft inflammatory factor 1 |
Synonyms: | AIF-1, IBA, IRT-1, IRT1, allograft, AIF-1, AIF1, G1, IBA1, IRT 1, Protein G1, Ionized calcium-binding adapter molecule 1, MRF1, Microglia response factor, BART 1, AIF1 protein |
Gene ID: | 199 |
Uniprot ID: | O70200 |
Immunogen: | Recombinant protein corresponding to Mouse Iba1 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Subcellular location: | Cytoskeleton, Ruffle membrane, Phagocytic cup |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 500-1: 4000 | brain |
Background
AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1.
Images
| IHC analysis of IBA-1 (GB12105). |
| IHC analysis of IBA-1 (GB12105). |
| Immunohistochemistry of paraffin embedded rat brain using IBA-1 (GB12105) at dilution of 1: 2000 (300x lens) |
| Immunofluorescent analysis of paraffin embedded rat hippocampus using Neun (GB11138) (green) + Iba1 (GB12105) (red) + MBP (GB12226) (spred) + GFAP (GB11096) (yellow) and Dapi in blue at dilution of 1: 1000 |
| Immunofluorescence of paraffin embedded mouse brain using IBA-1 (GB12105) at dilution of 1: 1000 (200x lens) |
| Immunofluorescent analysis of paraffin embedded rat brain using Neun (GB11138) (green)+ Iba1 (GB12105) (spred) + MAP2 (GB11128-2) (red) and Dapi in blue at dilution of 1: 1000 |
| IF analysis of IBA-1 (GB12105). |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
