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Anti-APG5L/ATG5 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | ATG5 rabbit polyclonal antibody |
Gene Name: | Autophagy protein 5 |
Synonyms: | ATG5, APG5, APG5-LIKE, APG5L ASP, hAPG5, autophagy related 5, SCAR25 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ATG5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm |
Uniprot ID: | Q9H1Y0, Q99J83 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, lung, lymph node, stomach |
Background
Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Images
 | Immunohistochemistry of paraffin embedded human kidney using ATG5 (GB11401) at dilution of 1:800 (400x lens) |
 | Immunohistochemistry of paraffin embedded human stomach using ATG5 (GB11401) at dilution of 1:1000 (400x lens) |
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Immunofluorescence of paraffin embedded mouse kidney using ATG5 (GB11401) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded mouse lung using ATG5 (GB11401) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded rat lymph node using ATG5 (GB11401) at dilution of 1:500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
