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| Reactivity: | M |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Serine/threonine-protein kinase ATR |
Synonyms: | ATR, FRAP related protein 1, FRP1, MEC1, SCKL, SCKL1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse ATR |
Uniprot ID: | Q9JKK8 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Chromosome |
Product Usage Information
IHC/IF | Mouse | 1: 100-1: 400 | testis, spleen, lung, liver, skeletal muscle |
Background
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication (By similarity). Positively regulates the restart of stalled replication forks following activation by the KHDC3-OOEP scaffold complex Essential for preventing the occurrence of DNA damage during early embryogenesis.
Images
| IHC analysis of ATR (GB115543). Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of ATR (GB115543). Sample: Mouse spleen (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of ATR (GB115543). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of ATR (GB115543). Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of ATR (GB115543). Sample: Mouse skeletal muscle (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
