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| Reactivity: | Human |
| Applications: | WB, IHC-P, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | BCL2 apoptosis regulator |
| Gene Symbol: | BCL2 |
| Synonyms: | Bcl-2; PPP1R50 |
| Gene ID: | 596 |
| UniProt ID: | P10415 |
| Clone ID: | 5T3F2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (P10415). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.49 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-BCL2 monoclonal antibody |
Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type (WT) and Bcl-2 knockdown (KD) 293T cells using [KD Validated] Bcl-2 Rabbit mAb (A21873) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human follicular lymphoma tissue using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg [KD Validated] Bcl-2 Rabbit mAb (A21873). Western blot was performed from the immunoprecipitate using [KD Validated] Bcl-2 Rabbit mAb (A21873) at a dilution of 1:1000. |
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