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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | BUB1 mitotic checkpoint serine/threonine kinase |
| Gene Symbol: | BUB1 |
| Synonyms: | BUB1A; BUB1L; hBUB1; MCPH30; [KD Validated] BUB1 |
| Gene ID: | 699 |
| UniProt ID: | O43683 |
| Clone ID: | 6U8G10 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human BUB1. (NP_004327.1). |
| Dilution: | WB 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.6 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-BUB1 monoclonal antibody |
Background
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from HEL cells, using [KD Validated] BUB1 Rabbit mAb (A23198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from wild type(WT) and BUB1 knockdown (KD) 293T cells, using [KD Validated] BUB1 Rabbit mAb (A23198) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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