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KD-Validated Calreticulin Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | calreticulin |
| Gene Symbol: | CALR |
| Synonyms: | RO; CRT; SSA; cC1qR; HEL-S-99n; [KD Validated] Calreticulin |
| Gene ID: | 811 |
| UniProt ID: | P27797 |
| Clone ID: | 10J1K8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-203 of human Calreticulin (NP_004334.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CALR monoclonal antibody |
Background
Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.
Images
 | Western blot analysis of various lysates using [KD Validated] Calreticulin Rabbit mAb (A20986) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type (WT) and Calreticulin knockdown (KD) 293T cells using [KD Validated] Calreticulin Rabbit mAb (A20986) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KD Validated] Calreticulin Rabbit mAb (A20986) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] Calreticulin Rabbit mAb (A20986) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] Calreticulin Rabbit mAb (A20986) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] Calreticulin Rabbit mAb (A20986) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] Calreticulin Rabbit mAb (A20986) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of Hep G2 cells using [KD Validated] Calreticulin Rabbit mAb (A20986,dilution 1:50)(Red) . DAPI was used for nuclear staining (blue). Objective: 40x. |
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