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| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Caudal type homeobox 2 |
| Gene Symbol: | CDX2 |
| Synonyms: | CDX3; CDX-3; CDX2/AS; CDX2 |
| Gene ID: | 1045 |
| UniProt ID: | Q99626 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 14-165 of human CDX2 (NP_001256.3). |
| Dilution: | WB 1:2000-1:8000; IHC 1:200-1:800; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 0.53mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CDX2 polyclonal antibody |
Background
This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.
Images
 | Western blot analysis of lysates from wild type (WT) and CDX2 knockdown (KD) 293T cells using CDX2 Rabbit pAb (A1629) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of Human colon cancer cells using CDX2 Rabbit pAb (A1629) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse colon cells using CDX2 Rabbit pAb (A1629) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of rat rectum cells using CDX2 Rabbit pAb (A1629) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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