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KD-Validated DIAPH1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | diaphanous related formin 1 |
| Gene Symbol: | DIAPH1 |
| Synonyms: | DIA1; DRF1; DFNA1; LFHL1; SCBMS; hDIA1; mDia1; [KD Validated] DIAPH1 |
| Gene ID: | 1729 |
| UniProt ID: | O60610 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1158-1272 of human DIAPH1 (NP_005210.3). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.65 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-DIAPH1 polyclonal antibody |
Background
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and DIAPH1 knockdown (KD) 293T cells, using [KD Validated] DIAPH1 Rabbit pAb (A18081) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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