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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | DNA methyltransferase 1 |
| Gene Symbol: | DNMT1 |
| Synonyms: | AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; [KD Validated] DNMT1 |
| Gene ID: | 1786 |
| UniProt ID: | P26358 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.84 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-DNMT1 polyclonal antibody |
Background
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells using [KD Validated] DNMT1 Rabbit pAb (A16729) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 3 s. |
 | Western blot analysis of lysates from Jurkat cells using [KD Validated] DNMT1 Rabbit pAb (A16729) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 3 s. |
 | Immunohistochemistry analysis of paraffin-embedded Human breast cancer using [KD Validated] DNMT1 Rabbit pAb (A16729) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KD Validated] DNMT1 Rabbit pAb (A16729) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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