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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | glycosylphosphatidylinositol specific phospholipase D1 |
| Gene Symbol: | GPLD1 |
| Synonyms: | PLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1; [KD Validated] GPLD1 |
| Gene ID: | 2822 |
| UniProt ID: | P80108 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2). |
| Dilution: | WB 1:1000-1:4000; IHC 1:500-1:2000; IF/IC 1:100-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.08 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GPLD1 polyclonal antibody |
Background
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
Images
 | Western blot analysis of lysates from Daudi cells, using [KD Validated] GPLD1 Rabbit pAb (A21781) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from wild type(WT) and GPLD1 knockdown (KD) 293T cells, using [KD Validated] GPLD1 Rabbit pAb (A21781) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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