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KD-Validated Gelsolin Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | gelsolin |
| Gene Symbol: | GSN |
| Synonyms: | ADF; AGEL; [KD Validated] Gelsolin |
| Gene ID: | 2934 |
| UniProt ID: | P06396 |
| Clone ID: | 0N10G5 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-782 of human Gelsolin (NP_000168.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.07 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GSN monoclonal antibody |
Background
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from Hep G2 cells, using [KD Validated] Gelsolin Rabbit mAb (A22184) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:200000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from wild type(WT) and Gelsolin knockdown (KD) HeLa cells, using [KD Validated] Gelsolin Rabbit mAb (A22184) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:200000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] Gelsolin Rabbit mAb (A22184) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] Gelsolin Rabbit mAb (A22184) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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