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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Histone deacetylase 1 |
| Gene Symbol: | HDAC1 |
| Synonyms: | HD1; RPD3; KDAC1; GON-10; RPD3L1; C1 |
| Gene ID: | 3065 |
| UniProt ID: | Q13547 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.32 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HDAC1 polyclonal antibody |
Background
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Images
 | Western blot analysis of various lysates using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:2000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 20 s. |
 | Western blot analysis of lysates from wild type (WT) and HDAC1 knockdown (KD) 293T cells, using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat spleen using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of A549 cells using [KD Validated] HDAC1 Rabbit pAb (A0238). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of L929 cells using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg [KD Validated] HDAC1 Rabbit pAb (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilution of 1:1000. |
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