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KD-Validated Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Integrin subunit alpha 5 |
| Gene Symbol: | ITGA5 |
| Synonyms: | FNRA; CD49e; VLA-5; VLA5A; [KD Validated] Integrin alpha 5 (ITGA5/CD49e) |
| Gene ID: | 3678 |
| UniProt ID: | P08648 |
| Clone ID: | 0S9S6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-995 of human Integrin alpha 5 (ITGA5/CD49e). (NP_002196.4). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.29 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ITGA5 monoclonal antibody |
Background
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Images
 | Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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