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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | actate dehydrogenase A |
| Gene Symbol: | LDHA |
| Synonyms: | LDHM; GSD11; PIG19; HEL-S-133P |
| Gene ID: | 3939 |
| UniProt ID: | P00338 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LDHA (NP_005557.1). |
| Dilution: | WB 1:2000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.8 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-LDHA monoclonal antibody |
Background
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Images
 | Western blot analysis of various lysates using [KD Validated] LDHA Rabbit mAb (A26493) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and LDHA knockdown (KD) 293T cells using [KD Validated] LDHA Rabbit mAb (A26493) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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