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| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NAD(P)H quinone dehydrogenase 1 |
| Gene Symbol: | NQO1 |
| Synonyms: | DTD; QR1; DHQU; DIA4; NMOR1; NMORI; [KD Validated] NQO1 |
| Gene ID: | 1728 |
| UniProt ID: | P15559 |
| Clone ID: | 7L9B4 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human NQO1 (NP_000894.1). |
| Dilution: | WB 1:1000-1:6000; IHC 1:200-1:2000; IF/IC 1:100-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.24 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NQO1 monoclonal antibody |
Background
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Images
 | Western blot analysis of various lysates, using [KD Validated] NQO1 Rabbit mAb (A22290) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Confocal imaging of paraffin-embedded human stomach using [KD Validated] NQO1 Rabbit mAb (A22290, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
 | Confocal imaging of paraffin-embedded mouse stomach using [KD Validated] NQO1 Rabbit mAb (A22290, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
 | Confocal imaging of paraffin-embedded rat stomach using [KD Validated] NQO1 Rabbit mAb (A22290, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining. |
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