KO-Validated AK2 Rabbit pAb (20 μl)

KO-Validated AK2 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A6519
Availability: In Stock
$128.00
-+
Reactivity: Human    
Applications: WB, IHC-P, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: adenylate kinase 2
Gene Symbol: AK2
Synonyms: ADK2; K2
Gene ID: 204
UniProt ID: P54819
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human AK2 (NP_001616.1).
Dilution: WB 1:500-1:1000
Purification Method: Affinity purification
Concentration: 2.56 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol ,pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-AK2 polyclonal antibody




Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.




Images

AK2 rabbit pAbWestern blot analysis of various lysates using [KO Validated] AK2 Rabbit pAb (A6519) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and AK2 knockout (KO) 293T cells, using [KO Validated] AK2 Rabbit pAb (A6519) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using [KO Validated] AK2 Rabbit pAb (A6519) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.



You may also be interested in:

AK2

Related Products


LifeSct © 2025

Publications

text_heading

Our Location

405 East Gude Dr., Suite 205, Rockville, MD 20850, USA

1-301-890-6420