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KO-Validated CD147/BSG Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | basigin (Ok blood group) |
| Gene Symbol: | BSG |
| Synonyms: | OK; 5F7; TCSF; CD147; EMPRIN; HAb18G; EMMPRIN; SG |
| Gene ID: | 682 |
| UniProt ID: | P35613 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 45-200 of human CD147/CD147/BSG (NP_940991.1). |
| Dilution: | WB 1:1000-1:12000; IHC 1:200-1:800; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.14 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-BSG polyclonal antibody |
Background
The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and CD147/CD147/BSG knockout (KO) 293T cells, using [KO Validated] CD147/BSG Rabbit pAb (A18032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from L-02 cells using CD147/BSG Rabbit pAb (A18032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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