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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | CD44 molecule (IN blood group) |
| Gene Symbol: | CD44 |
| Synonyms: | IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; H-CAM; HCELL; ECM-III; HUTCH-1; HUTCH-I; ECMR-III; Hermes-1; 44 |
| Gene ID: | 960 |
| UniProt ID: | P16070 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of human CD44 (NP_000601.3). |
| Dilution: | WB 1:1000-1:2000; IHC 1:1000-1:4000; IF/IC 1:100-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.74 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CD44 polyclonal antibody |
Background
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Images
 | Western blot analysis of various lysates using [KO Validated] CD44 Rabbit pAb (A12410) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type (WT) and CD44 knockout (KO) HeLa cells, using [KO Validated] CD44 Rabbit pAb (A12410) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of A431 cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:200. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of RAW264.7 cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (A12410) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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