KO-Validated Fatty Acid Synthase (FASN) Rabbit PolymAb® (20 μl)

Brand: Abclonal
Cat. #: A22462PM
Availability: In Stock

$148.00

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Description

Reactivity:	Human
Applications:	WB, IF/ICC, ELISA
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Monoclonal antibody
Gene Name:	Fatty acid synthase
Gene Symbol:	FASN
Synonyms:	FAS; OA-519; SDR27X1
Gene ID:	2194
UniProt ID:	P49327
Clone ID:	10F9J3
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) Rabbit PolymAb® (NP_004095.4).
Dilution:	WB 1:500-1:2000
Purification Method:	Affinity purification
Concentration:	0.48 mg/ml
Buffer:	PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-FASN monoclonal antibody

Background

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Images

	Western blot analysis of various lysates, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (FASN) antibody (A22462PM) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of lysates from wild type(WT) and Fatty Acid Synthase (FASN) knockout (KO) HeLa cells, using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HepG2 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using [KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® (A22462PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.