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KO-Validated Cleaved Gasdermin E (N terminal) Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | gasdermin E |
| Gene Symbol: | GSDME |
| Synonyms: | DFNA5; ICERE-1 |
| Gene ID: | 1687 |
| UniProt ID: | O60443 |
| Immunogen: | A synthetic peptide corresponding to residues surrounding Asp270 of human Gasdermin E(NP_004394.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.67 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GSDME monoclonal antibody |
Background
Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and Gasdermin E (N terminal) knockout (KO) HeLa cells using [KO Validated] Cleaved Gasdermin E (N terminal) Rabbit mAb (A26197) at 1:2000 dilution incubated at room temperature for 1.5 hours. Wild type (WT) and GSDME knockout (KO) HeLa cells were treated by TNF-α (50ng/mL) and CHX (10μg/mL) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. <b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b> |
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