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| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | glycogen synthase kinase 3 alpha |
| Gene Symbol: | GSK3A |
| Synonyms: | GSK3 alpha; GSK3A; 3α |
| Gene ID: | 2931 |
| UniProt ID: | P49840 |
| Clone ID: | 4I10Q9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GSK3α (P49840). |
| Dilution: | WB 1:5000-1:30000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GSK3A monoclonal antibody |
Background
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
Images
 | Western blot analysis of lysates from wild type (WT) and GSK3α knockout (KO) HeLa cells, using [KO Validated] GSK3α Rabbit mAb (A19060) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from Rat lung, using [KO Validated] GSK3α Rabbit mAb (A19060) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from Mouse brain, using [KO Validated] GSK3α Rabbit mAb (A19060) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg GSK3α antibody (A19060). Western blot was performed from the immunoprecipitate using GSK3α antibody (A19060) at a dilution of 1:1000. |
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