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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Histone deacetylase 1 |
| Gene Symbol: | HDAC1 |
| Synonyms: | HD1; RPD3; KDAC1; GON-10; RPD3L1 |
| Gene ID: | 3065 |
| UniProt ID: | Q13547 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 393-482 of human HDAC1 (NP_004955.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.41 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HDAC1 monoclonal antibody |
Background
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Images
 | Western blot analysis of lysates from NIH/3T3 cells using [KO Validated] HDAC1 Rabbit mAb (A26492) at 1:25000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type (WT) and HDAC1 knockout (KO) 293T cells using [KO Validated] HDAC1 Rabbit mAb (A26492) at 1:25000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KO Validated] HDAC1 Rabbit mAb (A26492) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
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