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KO-Validated Lsh/HELLS Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | helicase, lymphoid specific |
| Gene Symbol: | HELLS |
| Synonyms: | LSH; ICF4; PASG; SMARCA6; Nbla10143; LS |
| Gene ID: | 3070 |
| UniProt ID: | Q9NRZ9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 719-838 of human Lsh/HELLS (NP_060533.2). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.51 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HELLS polyclonal antibody |
Background
This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.
Images
 | Western blot analysis of various lysates using [KO Validated] Lsh/HELLS Rabbit pAb (A5831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and Lsh/HELLS knockout (KO) HeLa cells, using [KO Validated] Lsh/HELLS Rabbit pAb (A5831) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg Lsh/HELLS antibody (A5831). Western blot was performed from the immunoprecipitate using Lsh/HELLS antibody (A5831) at a dilution of 1:1000. |
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