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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | High mobility group box 1 |
| Gene Symbol: | HMGB1 |
| Synonyms: | HMG1; HMG3; HMG-1; SBP-1; B1 |
| Gene ID: | 3146 |
| UniProt ID: | P09429 |
| Clone ID: | 2C7P4 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (P09429). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.62 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HMGB1 monoclonal antibody |
Background
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Images
 | Western blot analysis of various lysates using [KO Validated] HMGB1 Rabbit mAb (A19529) at 1:6000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
 | Western blot analysis of lysates from wild type (WT) and HMGB1 knockout (KO) HeLa cells using [KO Validated] HMGB1 Rabbit mAb (A19529) at 1:6000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
 | Immunohistochemistry analysis of paraffin-embedded HeLa and HeLa-HBMG1-KO cells using [KO Validated] HMGB1 Rabbit mAb (A19529) at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Confocal imaging of NIH-3T3 cells using [KO Validated] HMGB1 Rabbit mAb (A19529,dilution 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x. |
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