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KO-Validated Cytokeratin 8 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | keratin 8 |
| Gene Symbol: | KRT8 |
| Synonyms: | K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; 8 |
| Gene ID: | 3856 |
| UniProt ID: | P05787 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 380-480 of human Cytokeratin 8 (NP_002264.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KRT8 polyclonal antibody |
Background
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
Images
 | Western blot analysis of lysates from Mouse large intestine, using Cytokeratin 8 Rabbit pAb (A24434) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type(WT) and Cytokeratin 8 knockout (KO) HeLa cells, using Cytokeratin 8 Rabbit pAb (A24434) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 0.5s. |
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