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Applications: | WB |
Host Species: | Rabbit |
Isotype: | IgG1 |
Conjugation: | Unconjugated |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of Actin(Plant Specific)(NP_190236.1). |
Dilution: | WB 1:1000-1:5000 |
Purification Method: | Affinity purification |
Concentration: | 0.45 mg/ml |
Buffer: | PBS with 0.09% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual |
Images
![]() | Western blot analysis of lysates from Oryza sativa using plant actin Rabbit pAb (AC053) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:(ECL Basic Kit (RM00020)). Exposure time: 180s. |