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Anti-Alkaline Phosphatase Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Alkaline Phosphatase rabbit polyclonal antibody |
Gene Name: | Alkaline phosphatase |
Synonyms: | Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, ALPL, BLAP, Bone Alkaline Phosphatase, NBAP |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Alkaline Phosphatase |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 60 kDa / 72 kDa |
Uniprot ID: | F8VPQ6 |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1500 | small intestine, placenta |
IHC/IF | Mouse, Rat | 1: 1500-1: 3000 | small intestine, duodenum |
Background
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut.
Images
| Western blot analysis of ALPI (GB113915) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat duodenum using ALPI (GB113915) at dilution of 1: 2100 |
| Immunofluorescence analysis of paraffin embedded mouse small intestine using ALPI (GB113915) at dilution of 1: 1500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
