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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | alanyl-tRNA synthetase 2, mitochondrial |
| Gene Symbol: | AARS2 |
| Synonyms: | AARSL; LKENP; COXPD8; MTALARS; MT-ALARS |
| Gene ID: | 57505 |
| UniProt ID: | Q5JTZ9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 491-620 of human AARS2 (NP_065796.2). |
| Dilution: | WB 1:1000-1:4000; IHC 1:100-1:500; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 2.03 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol ,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-AARS2 polyclonal antibody |
Background
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8.
Images
 | Western blot analysis of lysates from K-562 cells using [KD Validated] AARS2 Rabbit pAb (A25997) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and AARS2 knockdown (KD) 293T cells using [KD Validated] AARS2 Rabbit pAb (A25997) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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