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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, FCM |
| Isotype: | IgG |
| Clone: | 23GB1565 |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 |
| Gene Symbol: | ACAP2 |
| Synonyms: | CENTB2; CNT-B2 |
| Immunogen: | A synthesized peptide derived from human ACAP2 |
| Dilution: | WB 1:1,000-1:5,000; IF/IC 1:1,000; FCM 1:2,000 |
| Uniprot ID: | Q15057 |
| Gene ID: | 23527 |
| MW: | 88 kDa |
| Purification Source: | Cell culture |
| Purification Method: | Affinity Purified |
| Buffer: | Liquid in BSA, PH 7.4, 50% glycerol and less than 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze/thaw cycles. |
Background
The gene ACAP2 enables GTPase activator activity. Acts upstream of or within actin filament-based process. Located in ruffle.
Images
 | Western blotting analysis using anti-ACAP2 antibody. Total cell lysates(30 y g) from various cell lines were loaded and separated by SDs-PAGE. The blot was incubated with anti-ACAP2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. |
 | Western blotting analysis using anti-ACAP2 antibody. ACAP2 expression in wild type (WT) and ACAP2 shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. B-Tubulin serves as a loading control. The blot was incubated with anti-ACAP2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. . |
 | Flow cytometric analysis of ACAP2 expression in HT-1080 cells using ACAP2 antibody (1:2,000). Green, isotype control; red, ACAP2. |
 | Immunocytochemical staining of HT-1080 cells with ACAP2 antibody (1:1,000). Nuclei were stained blue with DAPI; ACAP2 was stained magenta with Alexa Fluor647. mages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 um. |
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