Your shopping cart is empty!
KD-Validated Argonaute-2 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | argonaute RISC catalytic component 2 |
| Gene Symbol: | AGO2 |
| Synonyms: | PPD; Q10; CASC7; EIF2C2; LESKRES; LINC00980 |
| Gene ID: | 27161 |
| UniProt ID: | Q9UKV8 |
| Clone ID: | 6V7O10 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 101-200 of human Argonaute-2 (NP_036286.2). |
| Dilution: | WB 1:2000-1:8000; IHC 1:600-1:6000; IF/IC 1:300-1:1200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.9 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-AGO2 monoclonal antibody |
Background
The gene AGO2 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type (WT) and Argonaute-2 knockdown (KD) HeLa cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at 1:2900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of various lysates using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at 1:2900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] Argonaute-2 Rabbit mAb (A25642) at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Confocal imaging of Hep G2 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of NIH/3T3 cells using [KD Validated] Argonaute-2 Rabbit mAb (A25642, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
You may also be interested in:
