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KD-Validated beta 2 Microglobulin Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC, IF/IC, FC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | beta-2-microglobulin |
| Gene Symbol: | B2M |
| Synonyms: | B2M; IMD43; beta-2-microglobulin; [KD Validated] beta 2 Microglobulin |
| Gene ID: | 567 |
| UniProt ID: | P61769 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human β2 Microglobulin(NP_004039.1). |
| Dilution: | WB 1:1000-1:4000; IHC 1:1000-1:4000; IF/IC 1:50-1:200; FC 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-B2M monoclonal antibody |
Background
The gene B2M encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Images
 | Western blot analysis of various lysates, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type(WT) and β2-microglobulin knockdown (KD) HeLa cells, using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using [KD Validated] beta 2 Microglobulin Rabbit mAb (A23430) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Flow cytometry:1X10^6 Daudi cells(negative control,left) and Hela cells (right) were surface-stained with [KD Validated] beta Microglobulin Rabbit mAb(A23430,2 μg/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,2 μg/mL,blue line).Non-fluorescently stained cells was used as blank control (red line). |
 | Flow cytometry:1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,2 μg/mL,left) or β2 Microglobulin Rabbit mAb(A23430,2 μg/mL,right). |
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