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KD-Validated COPB2 Rabbit PolymAb® (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | COPI coat complex subunit beta 2 |
| Gene Symbol: | COPB2 |
| Synonyms: | OPDD; MCPH19; beta'-COP |
| Gene ID: | 9276 |
| UniProt ID: | P35606 |
| Clone ID: | 7G4O9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2. (NP_004757.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.18 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-COPB2 monoclonal antibody |
Background
The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.
Images
 | Western blot analysis of various lysates, using [KD Validated] COPB2 Rabbit PolymAb® (A22646PM) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from wild type(WT) and COPB2 knockdown (KD) Hela cells, using [KD Validated] COPB2 Rabbit PolymAb® (A22646PM) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of HeLa cells using [KD Validated] COPB2 Rabbit PolymAb® (A22646PM) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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