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| Reactivity: | Human |
| Applications: | WB, IF/ICC, IHC-P, ChIP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | forkhead box K1 |
| Gene Symbol: | FOXK1 |
| Synonyms: | FOXK1L |
| Gene ID: | 221937 |
| UniProt ID: | P85037 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-733 of human FOXK1 (NP_001032242.1). |
| Purification Method: | Affinity purification |
| Concentration: | 1.27 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-FOXK1 monoclonal antibody |
Background
Enables 14-3-3 protein binding activity; DNA-binding transcription repressor activity, RNA polymerase II-specific; and transcription cis-regulatory region binding activity. Involved in several processes, including cellular glucose homeostasis; negative regulation of autophagy; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleus.
Images
 | Western blot analysis of lysates from wild type (WT) and FOXK1 knockdown (KD) HeLa cells using [KD Validated] FOXK1 mAb (A27583) at 1:6000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] FOXK1 mAb (A27583) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] FOXK1 mAb (A27583) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] FOXK1 mAb (A27583) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KD Validated] FOXK1 mAb (A27583) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Confocal imaging of HeLa cells using [KD Validated] FOXK1 mAb (A27583, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of NIH/3T3 cells using [KD Validated] FOXK1 mAb (A27583, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from 293F cells, using 3 μg of [KD Validated] FOXK1 mAb (A27583) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci. |
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