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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | lysine demethylase 2B |
| Gene Symbol: | KDM2B |
| Synonyms: | CXXC2; Fbl10; PCCX2; FBXL10; JHDM1B |
| Gene ID: | 84678 |
| UniProt ID: | Q8NHM5 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1059-1336 of human KDM2B (NP_115979.3). |
| Dilution: | WB 1:6000-1:24000; IF/IC 1:200-1:400; IHC 1:2000-1:8000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.94 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-KDM2B polyclonal antibody |
Background
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
Images
 | Western blot analysis of lysates from Hep G2 cells using [KD Validated] KDM2B Rabbit pAb (A25693) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 90 s. |
 | Western blot analysis of lysates from 293T cells using [KD Validated] KDM2B Rabbit pAb (A25693) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) Exposure time: 90 s. |
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