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| Reactivity: | Human |
| Applications: | WB, IF/IC, FCM |
| Isotype: | IgG |
| Clone: | 23GB2025 |
| Host Species: | Rabbit |
| Clonality: | Monoclonal antibody |
| Gene Name: | kinesin family member 11 |
| Gene Symbol: | KIF11 |
| Synonyms: | EG5; HKSP; KNSL1; MCLMR; TRIP5 |
| Immunogen: | A synthesized peptide derived from human Eg5 |
| Dilution: | WB 1:1,000-1:5,000; IF/IC 1:1,000; FCM 1:2,000 |
| Uniprot ID: | P52732 |
| Gene ID: | 3832 |
| MW: | 119 kDa |
| Purification Source: | Cell culture |
| Purification Method: | Affinity Purified |
| Buffer: | Liquid in BSA, PH 7.4, 50% glycerol and less than 0.02% sodium azide. |
| Storage: | Store at -20°C. Avoid repeated freeze/thaw cycles. |
Background
The gene KIF11 encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
Images
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Western blotting analysis using anti-Kinesin family member 11 antibody. Total cell lysates (30 ug) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Kinesin family member 11 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. |
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Western blotting analysis using anti-Kinesin family member 11 antibody. Kinesin family member 11 expression in wild type (WT) and kinesin family member 11shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. Hsp90 a serves as a loading control. The blot was incubated with anti-Kinesin family member 11 antibody(1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody(1:20,000) respectively. |
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Flow cytometric analysis of Kinesin family member 11 expression in HAP-1 cells using Kinesin family member 11 antibody (1:2,000). Green, isotype control; red, Kinesin family member 11. |
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lmmunocytochemical staining of HAP-1 cells with Kinesin family member 11antibody (1:1,000). Nuclei were stained blue with DAPl: Kinesin family member 11was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 um. |
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