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KD-Validated MAD1/MAD1L1 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | mitotic arrest deficient 1 like 1 |
| Gene Symbol: | MAD1L1 |
| Synonyms: | MAD1; MVA7; PIG9; TP53I9; TXBP181; [KD Validated] MAD1/MAD1L1 |
| Gene ID: | 8379 |
| UniProt ID: | Q9Y6D9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 351-718 of human MAD1/MAD1L1 (NP_003541.2). |
| Dilution: | WB 1:1000-1:6000; IHC 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.27 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MAD1L1 polyclonal antibody |
Background
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from HepG2 cells, using [KD Validated] MAD1/MAD1L1 Rabbit pAb (A22000) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from wild type(WT) and MAD1/MAD1L1 knockdown (KD) 293T cells, using [KD Validated] MAD1/MAD1L1 Rabbit pAb (A22000) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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