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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | microtubule associated protein 1 light chain 3 beta |
| Gene Symbol: | MAP1LC3B |
| Synonyms: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; 3B |
| Gene ID: | 81631 |
| UniProt ID: | Q9GZQ8 |
| Clone ID: | 1S3P9 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 2-107 of human LC3B (Q9GZQ8). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1 mg/ml |
| Buffer: | PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MAP1LC3B monoclonal antibody |
Background
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Images
 | Western blot analysis of various lysates, using [KD Validated] LC3B Rabbit mAb (A19665) at 1:1000 dilution. 293T, C6 and NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Western blot analysis of lysates from wild type(WT) and LC3B knockdown (KD) 293T cells, using [KD Validated] LC3B Rabbit mAb (A19665) at 1:1000 dilution. wild type(WT) and LC3B knockdown (KD) 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using [KD Validated] LC3B Rabbit mAb (A19665) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Confocal imaging of HeLa cells (treated with Chloroquine) and HeLa cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of C6 cells (treated with Chloroquine) and C6 cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of NIH/3T3 cells (treated with Chloroquine) and NIH/3T3 cells (untreated) using [KD Validated] LC3B Rabbit mAb (A19665, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Immunoprecipitation analysis of 300 μg extracts from 293T cells using 3 μg [KD Validated] LC3B Rabbit mAb (A19665). Western blot was performed from the immunoprecipitate using LC3B antibody (A19665) at a dilution of 1:1000. |
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