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| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | microtubule associated protein 1 light chain 3 beta |
| Gene Symbol: | MAP1LC3B |
| Synonyms: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; 3B |
| Gene ID: | 81631 |
| UniProt ID: | Q9GZQ8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human LC3B (NP_073729.1). |
| Dilution: | WB 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MAP1LC3B polyclonal antibody |
Background
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Images
 | Western blot analysis of various lysates using [KD Validated] LC3B Rabbit pAb (A7198) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of lysates from wild type (WT) and LC3B knockdown (KD) 293T,293T+chloroquine cells, using [KD Validated] LC3B Rabbit pAb (A7198) at 1:1000 dilution. 293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human breast cancer using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human stomach using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of paraffin-embedded rat brain using [KD Validated] LC3B Rabbit pAb (A7198) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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