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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | malic enzyme 1 |
| Gene Symbol: | ME1 |
| Synonyms: | MES; HUMNDME; [KD Validated] ME1 |
| Gene ID: | 4199 |
| UniProt ID: | P48163 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 472-572 of human ME1. (NP_002386.1). |
| Dilution: | WB 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 0.34 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-ME1 polyclonal antibody |
Background
This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
Images
 | Western blot analysis of lysates from wild type(WT) and ME1 knockdown (KD) MCF7 cells, using [KD Validated] ME1 Rabbit pAb (A23068) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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