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KD-Validated METTL14 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | methyltransferase 14, N6-adenosine-methyltransferase non-catalytic subunit |
| Gene Symbol: | METTL14 |
| Synonyms: | hMETTL14; [KD Validated] METTL14 |
| Gene ID: | 57721 |
| UniProt ID: | Q9HCE5 |
| Clone ID: | 5R1C3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.94 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-METTL14 monoclonal antibody |
Background
Enables mRNA binding activity. Contributes to mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity. Involved in mRNA metabolic process; negative regulation of hematopoietic progenitor cell differentiation; and positive regulation of translation. Located in nucleoplasm. Part of RNA N6-methyladenosine methyltransferase complex.
Images
 | Western blot analysis of various lysates using [KD Validated] METTL14 Rabbit mAb (A24396) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
 | Western blot analysis of lysates from wild type (WT) andMETTL14 knockdown (KD) A549 cells using [KD Validated] METTL14 Rabbit mAb (A24396) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] METTL14 Rabbit mAb (A24396) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] METTL14 Rabbit mAb (A24396) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] METTL14 Rabbit mAb (A24396) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis using [KO Validated] METTL14 Rabbit mAb (A24396) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunoprecipitation of METTL14 from 300 µg extracts of Raji cells was performed using 3 µg of METTL14 Rabbit mAb (A24396). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using METTL14 Rabbit mAb (A24396) at a dilution of 1:500. |
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