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KD-Validated METTL3 Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IP, CHIP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit |
| Gene Symbol: | METTL3 |
| Synonyms: | M6A; IME4; Spo8; MT-A70; hMETTL3 |
| Gene ID: | 56339 |
| UniProt ID: | Q86U44 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human METTL3 (NP_062826.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.3 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-METTL3 monoclonal antibody |
Background
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Images
 | Western blot analysis of various lysates using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of lysates from Rat brain using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from wild type (WT) and METTL3 knockdown (KD) 293T cells using [KD Validated] METTL3 Rabbit mAb (A26858) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using [KD Validated] METTL3 Rabbit mAb (A26858) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
 | Immunoprecipitation of METTL3 from 300 µg extracts of HepG2 cells was performed using 0.5 µg of [KD Validated] METTL3 Rabbit mAb (A26858). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KD Validated] METTL3 Rabbit mAb (A26858) at a dilution of 1 : 1000. |
 | Immunoprecipitation of METTL3 from 300 µg extracts of HepG2 cells was performed using 0.5 µg of METTL3 Rabbit mAb (A26858). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using METTL3 Rabbit mAb (A26858) at a dilution of 1:1000. |
 | Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from MCF7, using 3 μg of [KD Validated] METTL3 Rabbit mAb (A26858) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci. |
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