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KD-Validated MRPL52 Rabbit pAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | mitochondrial ribosomal protein L52 |
| Gene Symbol: | MRPL52 |
| Synonyms: | mL52, 39S ribosomal protein L52, L52mt, MRP-L52, Mrpl52, RM52 |
| Gene ID: | 122704 |
| UniProt ID: | Q86TS9 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 24-123 of human MRPL52 (NP_848026.1). |
| Purification Method: | Affinity purification |
| Concentration: | 1.55 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-MRPL52 polyclonal antibody |
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which has no bacterial homolog. Multiple transcript variants encoding different protein isoforms were identified through sequence analysis.
Images
 | Western blot analysis of lysates from wild type (WT) and MRPL52 knockdown (KD) HeLa cells using [KD Validated] MRPL52 Rabbit pAb (A25498) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
 | Western blot analysis of lysates from U-251 MG cells using [KD Validated] MRPL52 Rabbit pAb (A25498) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:45s. |
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