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| Reactivity: | Human |
| Applications: | WB, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | nicotinamide N-methyltransferase |
| Gene Symbol: | NNMT |
| Synonyms: | Nicotinamide N-methyltransferase |
| Gene ID: | 4837 |
| UniProt ID: | P40261 |
| Clone ID: | 1R5Z1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human NNMT (NP_006160.1). |
| Dilution: | WB 1:3000-1:12000; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 1.84 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-NNMT monoclonal antibody |
Background
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
Images
 | Western blot analysis of lysates from wild type (WT) and NNMT knockdown (KD) HeLa cells using [KD Validated] NNMT Rabbit mAb (A25391) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Confocal imaging of A549 cells using [KD Validated] NNMT Rabbit mAb (A25391, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Immunoprecipitation of NNMT in 200 µg extracts from A549 cells using 0.5 µg [KD Validated] NNMT Rabbit mAb (A25391). Western blot analysis was performed using [KD Validated] NNMT Rabbit mAb (A25391) at 1:3000 dilution. |
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